Ascorbic Acid Quantification in Melon Samples – the Importance of the Extraction Medium for Hplc Analysis
نویسندگان
چکیده
Two high-performance liquid chromatography (HPLC) methods for ascorbic acid (AA) quantification were compared in three cultivars of melon (Cucumis melo L.) fruits. Both procedures were analogous except for the extraction medium used: 6 % meta-phosphoric acid and 3 % citric acid. The general conclusion is that 3 % citric acid is a better extraction medium compared to 6 % meta-phosphoric acid and it can be used for ascorbic acid quantification analyses in melon samples.
منابع مشابه
A Dispersive Liquid–Liquid Micro–Extraction Technique for the Pre–concentration and Quantification of Vitamin D3 in Milk and Yogurt Samples Using a Non-Aqueous HPLC Method
In present study, a DLLME-HPLC-UV method was developed and validated for the extraction, pre–concentration, and subsequently quantification of vitamin D3 (Vit D3) in milk and yogurt samples. In order to be able to extract Vit D3 from studied samples efficiently, the DLLME procedure was optimized with respect to the parameters affecting the extraction efficacy, w...
متن کاملIn vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)
In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...
متن کاملA Dispersive Liquid–Liquid Micro–Extraction Technique for the Pre–concentration and Quantification of Vitamin D3 in Milk and Yogurt Samples Using a Non-Aqueous HPLC Method
In present study, a DLLME-HPLC-UV method was developed and validated for the extraction, pre–concentration, and subsequently quantification of vitamin D3 (Vit D3) in milk and yogurt samples. In order to be able to extract Vit D3 from studied samples efficiently, the DLLME procedure was optimized with respect to the parameters affecting the extraction efficacy, w...
متن کاملIn vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)
In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...
متن کاملDetermination of Vitamin C in Small Volumes of Blood by HPLC/EC
A sensitive procedure for determining total vitamin C (ascorbic acid + dehydrate ascorbic Acid) in a blood drop from a finger prick, before and after the adminis- tration of a vitamin C tablet is described. Analysis was carried out by high per- formance liquid chromatography with electrochemical detection (HPLC/EC). Measurements were taken one hour, two hours and six hours after the adm...
متن کامل